توضیحات
This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope. Read more…
Abstract: This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope
این جلد پروتکلهایی را که زرادخانه وسیعی از تکنیکهای مورد استفاده برای مطالعه یک سیستم ترشحی از A تا Z را پوشش میدهد، توضیح میدهد. مسیر مونتاژ توسط میکروسکوپ فلورسانس و نقش انرژی در طول مونتاژ و/یا ترشح، شناسایی عوامل ترشح شده و همچنین گزارشگران برای دنبال کردن انتقال اثر. این فصلها که در قالبهای بسیار موفق سری Methods in Molecular Biology نوشته شدهاند، شامل مقدمهای بر موضوعات مربوطه، فهرستی از مواد و معرفهای لازم، پروتکلهای آزمایشگاهی گام به گام و بهراحتی قابل تکرار و نکاتی در مورد عیبیابی و اجتناب از دامهای شناخته شده است. سیستمهای ترشح پروتئین باکتریایی معتبر و پیشرفته: روشها و پروتکل با هدف ارائه تکنیکهایی است که به مطالعه سیستمهای ترشحی محدود نمیشود، بلکه برای هر محققی که علاقهمند به کمپلکسهای چند پروتئینی پوشش سلولی باکتری است، مورد توجه خاص است. /span> بیشتر بخوانید…
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